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The Resource Spectroscopic study of compressible mobile phase and stationary phase behavior in chromatography, by Lawrence Robert Baker

Spectroscopic study of compressible mobile phase and stationary phase behavior in chromatography, by Lawrence Robert Baker

Label
Spectroscopic study of compressible mobile phase and stationary phase behavior in chromatography
Title
Spectroscopic study of compressible mobile phase and stationary phase behavior in chromatography
Statement of responsibility
by Lawrence Robert Baker
Creator
Subject
Genre
Language
eng
Summary
Raman spectroscopy, laser-induced fluorescence, and sum-frequency generation (SFG) spectroscopy are used to investigate the behavior of compressible mobile phases and stationary phases under a variety of chromatographic conditions. Efforts to understand and optimize separations employing compressible mobile phases have been limited by a lack of understanding of the mobile phase density gradient. Mobile phase compressibility leads to gradients in linear velocity and solute retention and affects separation speed and efficiency, especially in packed columns. This work describes on-column density measurement of CO2, a common carrier fluid for SFC and SGC, in packed capillary columns using Raman microspectroscopy. On-column detection by laser-induced fluorescence is used to observe the effect of the mobile phase density gradient on separation speed and efficiency, and experimental efficiency is compared to a theoretical model. Additionally, SFG spectroscopy allows for probing the structure of model monomeric and polymeric C18 stationary phases under pressure; this provides a basis for correlating selectivity with pressure-induced structural changes in stationary phase materials. Together, this work provides a more complete understanding of the role of column pressure and fluid compressibility on the speed, efficiency, and selectivity of chemical separations
Cataloging source
UPB
Degree
M.S.
Dissertation year
2008
Granting institution
Brigham Young University. Dept. of Chemistry and Biochemistry
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • bibliography
  • theses
Label
Spectroscopic study of compressible mobile phase and stationary phase behavior in chromatography, by Lawrence Robert Baker
Link
http://hdl.lib.byu.edu/1877/etd2581
Instantiates
Publication
Bibliography note
Includes bibliographical references
Carrier category
volume
Carrier MARC source
rdacarrier
Content category
text
Content type MARC source
rdacontent
Dimensions
28 cm.
Extent
viii, 115 p.
Media category
unmediated
Media MARC source
rdamedia
Other physical details
ill. (some col.)
System control number
  • UtOrBLW
  • (OCoLC)ocn373798897
Label
Spectroscopic study of compressible mobile phase and stationary phase behavior in chromatography, by Lawrence Robert Baker
Link
http://hdl.lib.byu.edu/1877/etd2581
Publication
Bibliography note
Includes bibliographical references
Carrier category
volume
Carrier MARC source
rdacarrier
Content category
text
Content type MARC source
rdacontent
Dimensions
28 cm.
Extent
viii, 115 p.
Media category
unmediated
Media MARC source
rdamedia
Other physical details
ill. (some col.)
System control number
  • UtOrBLW
  • (OCoLC)ocn373798897

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