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The Resource Studies on cyclooxygenase-1, its splice variants, and modulation of cyclooxygenase-2 by inducible nitric oxide synthase and novel phytochemicals, by Yibing Xu

Studies on cyclooxygenase-1, its splice variants, and modulation of cyclooxygenase-2 by inducible nitric oxide synthase and novel phytochemicals, by Yibing Xu

Label
Studies on cyclooxygenase-1, its splice variants, and modulation of cyclooxygenase-2 by inducible nitric oxide synthase and novel phytochemicals
Title
Studies on cyclooxygenase-1, its splice variants, and modulation of cyclooxygenase-2 by inducible nitric oxide synthase and novel phytochemicals
Statement of responsibility
by Yibing Xu
Creator
Author
Subject
Language
eng
Summary
  • Cyclooxygenases (COXs) are of important therapeutic value as they are the target site of aspirin-like drugs. Here I report nine new COX-1 splice variants in chapter 1, which I characterized with regard to heme-binding and other properties
  • Inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) are co-inducible in many tissues following mitogenic and proinflammatory stimulation. In chapter 2, I investigate the physical and enzymatic properties of human COX-2 and iNOS and demonstrate that, despite reports to the contrary by another laboratory, they do not interact
  • The only reported COX-1 splice variant to exhibit cyclooxygenase activity has been isolated from dog brain and is termed COX-3. It contains an in-frame insertion of intron 1. However the existence of human COX-3 remains questionable since intron 1 is out of frame. Two putative in-frame human COX-3 isozymes, COX-1b2 and COX-1b3, (herein designated as COX-3-72 and COX-3-50) have been reported in the literature, but only one of them, COX-3-72, has been characterized. In chapter 3, COX-3-50 and COX-3-72 are reported to be over-expressed and determined to be active cyclooxygenases. COX-3-72 and, to a greater extent, COX-3-50, were stimulated by rofecoxib at physiological concentrations. A similar rofecoxib-stimulated COX activity is observed in quiescent A549 cells. Immunoblot and immunoprecipitation analysis suggest that human platelet and potentially A549 cells, contain a COX-3-50 like protein
  • Lonicera japonica is used as an anti-inflammatory treatment in traditional Chinese medicine. Its working mechanism is not well known. In chapter 4, I report that extracts from this herb inhibit COX-2 by three mechanisms: direct inhibition, transcriptional and post-transcriptional down regulation
  • COX-1 and COX-2 are similar to each other in their crystallographic structures. One of the most striking differences is that there are eight amino acids immediately following the signal peptide in COX-1 which are not found in COX-2. The function of this sequence is unknown. In chapter 5, I found that deletion of these amino acids decreased COX-1 Vmax by approximately 4-fold, but had little effect on other properties of the enzyme
  • Selecting bacteria transformed with recombinant plasmids is a laborious step in gene cloning experiments. This selection process is even more tedious when large numbers of clones need to be screened. In appendix I, I describe an ultra fast plasmid screening method. This new method was frequently used in the experiments performed in chapters 2-6
Cataloging source
UPB
Degree
Doctor of Philosophy
Dissertation year
2006
Granting institution
Brigham Young University. Department of Chemistry and Biochemistry
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • bibliography
  • theses
Label
Studies on cyclooxygenase-1, its splice variants, and modulation of cyclooxygenase-2 by inducible nitric oxide synthase and novel phytochemicals, by Yibing Xu
Instantiates
Publication
Bibliography note
Includes bibliographical references
Carrier category
volume
Carrier MARC source
rdacarrier
Content category
text
Content type MARC source
rdacontent
Dimensions
28 cm.
Extent
xi, 102 p.
Media category
unmediated
Media MARC source
rdamedia
Other physical details
ill. (some col.)
System control number
  • UtOrBLW
  • UtOrBLW
  • (OCoLC)ocn373829498
Label
Studies on cyclooxygenase-1, its splice variants, and modulation of cyclooxygenase-2 by inducible nitric oxide synthase and novel phytochemicals, by Yibing Xu
Publication
Bibliography note
Includes bibliographical references
Carrier category
volume
Carrier MARC source
rdacarrier
Content category
text
Content type MARC source
rdacontent
Dimensions
28 cm.
Extent
xi, 102 p.
Media category
unmediated
Media MARC source
rdamedia
Other physical details
ill. (some col.)
System control number
  • UtOrBLW
  • UtOrBLW
  • (OCoLC)ocn373829498

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